Journal: Cancer cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity.

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet: Figure 4. Phenotypic plasticity of mesenchymal marker expression (A) Flow cytometry analysis of PDPN and CD105 in purified and in-vitro-cultured CD105pos and CD105neg pancreatic fibroblasts (PaFs) after 1 and 7 weeks. Plots are representative of n = 4 experiments. Relative frequencies shown in relevant quadrants. (B) Normalized Eng mRNA expression in purified CD105pos (n = 4) and CD105neg PaFs (n = 4) treated with control (top) or KPC PDA conditioned medium (bottom). Data displayed as mean ± SD. (C) Representative flow cytometry analysis (n = 4) of CD105 on GFPposCD105pos and GFPposCD105neg PaFs in mono- or co-culture with RFPpos KPC PDA tu- mor cells. (D) Representative flow cytometry analysis (n = 3) of CD105 in isolated CD105pos and CD105neg human PaFs after >3 weeks of in vitro culture. (E and F) MC analysis of primary PaFs treated with the indicated ligands for 3 days. Representative plots displaying relative frequencies of CD105pos and CD105neg PaFs. (G and H) Heatmap of median marker intensity (MMI) displayed as column Z scores for each phenotypic marker on CD105pos (G) and CD105neg (H) PaFs after 3 days of treatment as indicated. Boxplots show MMI with upper and lower boundary of the interquartile range and whiskers denoting maximum and minimum values minus outliers, across all conditions. (I and J) Representative flow cytometry analysis (n = 3) of CD105pos (I) and CD105neg (J) PaFs with IFN-g, IFN-g + KPC PDA conditioned medium, or IFN-g + TGF- b1 treatment. Samples are compared using unpaired t tests (B) (top and bottom). ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001. See also Figure S5 and Table S4.

Article Snippet: The first staining round used mouse anti-human CD105 antibody (CST clone 3A9) at 1/200 and TSA570 (FP1488001KT).

Techniques: Marker, Expressing, Flow Cytometry, In Vitro, Cell Culture, Control, Cytometry, Co-Culture Assay, Isolation

Journal: Oncotarget

Article Title: New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams

doi: 10.18632/oncotarget.12456

Figure Lengend Snippet: ( A ) Representative image of BM-MSCs 5 days after seeding. ( B – D ) Characterization of BM-MSCs by flow cytometry. The majority of the cells are CD105 + , CD44 + and CD29 + , which are typical characteristic phenotypes of BM-MSCs. ( E ) BM-MSC can be differentiated into adipogenic and myogenic lineages. Adipogenic differentiation was characterized by Oil Red O staining, while myogenic differentiation was evidenced by the formation of myotubes stained with crystal violet. ( F ) Representative image of immunostaining of BM-MSCs on GF scaffold, stained by anti-β-tubulin (green) and DAPI for nucleus (blue). Effects of different concentrations of VC (5 to 100 μg/ml, 5 days) ( G ) and H 2 O 2 exposure (0.1 to 2 mM, 24 hours) ( H ) on cell viability of BM-MSCs cultured for 5 days, measured by MTT assay. ( I ) Cell viability of BM-MSCs in the five experimental groups. Data were presented as mean ± SEM. * p < 0.05 vs control.

Article Snippet: Antibodies used in the study were as follows: CD105-FITC (MCA1557FT, Serotec, Oxford, UK), CD44-FITC (MCA643FA, Serotec), and CD29-FITC (MCA1949FT, Serotec).

Techniques: Flow Cytometry, Staining, Immunostaining, Cell Culture, MTT Assay, Control